SubtiBank SubtiBank
ylbB [2019-02-19 19:52:31]
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ylbB [2019-02-19 19:52:31]

putative oxidoreductase
Locus
BSU14950
Isoelectric point
4.76
Molecular weight
16.21 kDa
Protein length
148 aa Sequence Blast
Gene length
447 bp Sequence Blast
Function
unknown
Product
unknown
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other
  • Groups of genes → Poorly characterized/ putative enzymes

    • Gene

    Coordinates
    1,565,849 1,566,295

    The protein

    Paralogous protein(s)

  • YhcV:

    • Domains

  • 2 CBS domains (8-68, 74-130)

    • Structure

  • 4FRY (protein from Burkholderia ambifaria, 38% identity) PubMed

    • Expression and Regulation

    Operons

    Genes
    ylbB-ylbC
    Description
    PubMed

    Sigma factors

  • SigF: sigma factor, PubMed, in SigF regulon

    • Regulation

  • expressed during sporulation in the forespore (SigF) PubMed
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    Biological materials

    Mutant

  • MGNA-B242 (ylbB::erm), available at the NBRP B. subtilis, Japan
  • BKE14950 (ylbB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCGTTTAACCTCCTTTA, downstream forward: _UP4_TAACACATAAATCTTTGACA
  • BKK14950 (ylbB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCGTTTAACCTCCTTTA, downstream forward: _UP4_TAACACATAAATCTTTGACA

    • Expression vectors

  • pGP2934 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jrg Stlke's lab

    • References

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